- Several paintings by Caravaggio including the majestic Entombment
- Leonardo da Vinci’s portrait of Saint Jerome
- Works by painters Fra Angelico, Giotto, Raphael, Nicolas Poussin and Titian
- The red marble papal throne, formerly in the Basilica di San Giovanni in Laterano
- Roman sculpture, tombstones, and inscriptions, including the Early Christian Sarcophagus of Junius Bassus and Dogmatic sarcophagus, and the epitaph of Lucius Cornelius Scipio Barbatus
- The Raphael Rooms
- The Niccoline Chapel
- The Sistine Chapel
- The Gallery of Maps: topographical maps of the whole of Italy, painted on the walls by friar Ignazio Danti of Perugia
- The frescoes and other works in the Borgia Apartment built for Pope Alexander VI …

1/  Louvre, Paris (8,300,000,  visitors 2007)
2/  Centre Pompidou, Paris (5,509,425 )
3/ Tate Modern, London (5,191,840 )
4/ British Museum, London (4,837,878 )
5/ Metropolitan Museum of Art, New York (4,547,353)
6/ National Gallery of Art, Washington (4,518,413)
7/ Vatican Museums, Vatican City (4,310,083)
8/ National Gallery London (4,100,000)
9/ Musée d’Orsay, Paris (3,166,509)
10/ Museo Nacional del Prado, Madrid (2,652,924)
11/ National Palace Museum, Taipei (2,650,551)
12/ Victoria and Albert Museum, London (2,435,300)
13/ State Hermitage Museum, St Petersburg (2,395,075)
14/ Kelvingrove Art Gallery and Museum, Glasgow (2,232,475)
15/ Museum of Modern Art, New York (2,219,554)
16/ Field Museum, Chicago (2,133,149)
17/ Tokyo National Museum, Tokyo (1,772,255)
18/ CaxiaForum Barcelona, Barcelona (1,674,607)
19/ Moscow Kremlin Museums, Moscow (1,650,000)
20/ Museum of Fine Arts, Houston (1,649,960)